A rapid versatile microassay for cellular retinol-binding protein using Lipidex-1000 microcolumns.
A new, rapid and versatile microassay for cellular retinol-binding protein has been developed based on separation of bound and free ligand by means of Lipidex-1000, a hydrophobic Sephadex derivative.This requires quantitative manipulation of retinol in aqueous solution.The tendency of CoQ10 retinol to adhere to glass and plastic surfaces was overco